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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USH1G Polyclonal antibody

USH1G Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 21936-1-AP

Synonyms

ANKS4A, SANS, USH1G, Usher syndrome type 1G protein

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using USH1G Polyclonal antibody (21936-1-AP)

    IP experiment of NIH/3T3 using 21936-1-AP

    IP result of anti-USH1G (IP:21936-1-AP, 4ug; Detection:21936-1-AP 1:500) with NIH/3T3 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USH1G Polyclonal antibody (21936-1-AP)

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USH1G Polyclonal antibody (21936-1-AP)

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USH1G Polyclonal antibody (21936-1-AP)

    IHC staining of mouse brain using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USH1G Polyclonal antibody (21936-1-AP)

    IHC staining of mouse brain using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21936-1-AP is USH1G Fusion Protein expressed in E. coli.

Tested Reactivity Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USH1G fusion protein Ag16571 相同性解析による交差性が予測される生物種
Full Name Usher syndrome 1G (autosomal recessive)
Calculated molecular weight 461 aa, 51 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC101099
Gene symbol USH1G
Gene ID (NCBI) 124590
RRIDAB_2878950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for USH1G antibody 21936-1-APDownload protocol
IP protocol for USH1G antibody 21936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cells

Nuclear-Cytoplasmic Shuttling of the Usher Syndrome 1G Protein SANS Differs from Its Paralog ANKS4B

Authors - Jacques S Fritze
  • KD Validated
View Article