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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USMG5 Polyclonal antibody

USMG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17716-1-AP

Synonyms

HCV F transactivated protein 2, HCVFTP2, USMG5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis using 17716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis of human brain using 17716-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis of human liver using 17716-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of human placenta using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of human placenta using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human liver tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17716-1-AP targets USMG5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USMG5 fusion protein Ag12086 相同性解析による交差性が予測される生物種
Full Name up-regulated during skeletal muscle growth 5 homolog (mouse)
Calculated molecular weight 58 aa, 7 kDa
Observed molecular weight 6 kDa
GenBank accession numberBC072683
Gene symbol USMG5
Gene ID (NCBI) 84833
RRIDAB_2214288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for USMG5 antibody 17716-1-APDownload protocol
IHC protocol for USMG5 antibody 17716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.

Authors - James B Papizan
View Article
mouseIF

Sci Rep

miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy.

Authors - M Sanson
View Article
ratWB

Mol Psychiatry

Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala

Authors - Kayla Farrell
View Article