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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP10 Monoclonal antibody (67917-1-Ig)

    WB analysis using 67917-1-Ig (same clone as 67917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67917-1-Ig (USP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using USP10 Monoclonal antibody (67917-1-Ig)

    WB analysis of MCF-7 using 67917-1-Ig (same clone as 67917-1-PBS)

    WB result of USP10 antibody (67917-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP10 Monoclonal antibody (67917-1-Ig)

    IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP10 Monoclonal antibody (67917-1-Ig)

    IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using USP10 Monoclonal antibody (67917-1-Ig)

    IF Staining of HEK-293 using 67917-1-Ig (same clone as 67917-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (67917-1-Ig, Clone: 1B10C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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USP10 Monoclonal antibody, PBS Only

USP10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67917-1-PBS
Clone No.1B10C9

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67917-1-PBS targets USP10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP10 fusion protein Ag7233 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 10
Calculated molecular weight 87 kDa, 92 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC000263
Gene Symbol USP10
Gene ID (NCBI) 9100
RRIDAB_2918671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14694
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)