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  • Featured Product
  • KD/KO Validated

USP11 Polyclonal antibody, PBS Only

USP11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10244-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using USP11 Polyclonal antibody (10244-1-AP)

    WB analysis of Jurkat using 10244-1-AP (same clone as 10244-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using USP11 Polyclonal antibody (10244-1-AP)

    WB analysis of human testis using 10244-1-AP (same clone as 10244-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP11 Polyclonal antibody (10244-1-AP)

    WB analysis of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using USP11 Polyclonal antibody (10244-1-AP)

    IP experiment of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

    IP result of anti-USP11 (IP:10244-1-AP, 4ug; Detection:10244-1-AP 1:300) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP11 Polyclonal antibody (10244-1-AP)

    IHC staining of human colon cancer using 10244-1-AP (same clone as 10244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10244-1-AP (USP11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10244-1-PBS targets USP11 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP11 fusion protein Ag0296 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC000350
Gene symbol USP11
Gene ID (NCBI) 8237
RRIDAB_2288400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.