Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 22340-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22340-1-AP
WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.
WB analysis of A549 using 22340-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 22340-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 22340-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22340-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22340-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 22340-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 22340-1-AP
IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug.
IHC staining of human breast cancer using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 22340-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 22340-1-AP
Immunofluorescent analysis of HEK-293 cells using 22340-1-AP (USP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

USP11 Polyclonal antibody

USP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22340-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, A549 cells, HEK-293 cells, human testis tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human brain tissue, human liver cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

22340-1-AP targets USP11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP11 fusion protein Ag17829 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 11
Calculated molecular weight	963 aa, 110 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC000350
Gene Symbol	USP11
Gene ID (NCBI)	8237
RRID	AB_2879079
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51784
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.

Protocols

Product Specific Protocols
WB protocol for USP11 antibody 22340-1-AP	Download protocol
IHC protocol for USP11 antibody 22340-1-AP	Download protocol
IF protocol for USP11 antibody 22340-1-AP	Download protocol
IP protocol for USP11 antibody 22340-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Lab Invest Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma. Authors - Sheng Zhang KD Validated View Article
	WB	Cell Rep TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Authors - Qingyu Luo View Article
human	WB,IF	Cell Death Discov Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis. Authors - Yang Gao View Article
human	WB,IF	Cell X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women Authors - Yan Yan KD Validated View Article
human	WB	Sci Adv Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia Authors - Qi Jin KD Validated View Article
human	IHC,IF	Cell Death Dis USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1 Authors - Junyi Duan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22340-1-AP

WB analysis of HEK-293 using 22340-1-AP

WB analysis of A549 using 22340-1-AP

WB analysis of human testis using 22340-1-AP

WB analysis of Jurkat using 22340-1-AP

WB analysis of HEK-293 using 22340-1-AP

WB analysis of HEK-293 using 22340-1-AP

WB analysis of human testis using 22340-1-AP

IP experiment of HEK-293 using 22340-1-AP

IHC staining of human breast cancer using 22340-1-AP

IHC staining of human breast cancer using 22340-1-AP

IHC staining of human liver cancer using 22340-1-AP

IHC staining of human liver cancer using 22340-1-AP

IHC staining of human testis using 22340-1-AP

IHC staining of human testis using 22340-1-AP

IHC staining of human brain using 22340-1-AP

IHC staining of human brain using 22340-1-AP

IF Staining of HEK-293 using 22340-1-AP

USP11 Polyclonal antibody

USP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women

Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia

USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1