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USP12 Polyclonal antibody
USP12 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 12608-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
12608-1-AP targets USP12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP12 fusion protein Ag3309 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 12 |
| Calculated molecular weight | 370 aa, 43 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC026072 |
| Gene symbol | USP12 |
| Gene ID (NCBI) | 219333 |
| RRID | AB_10641037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ubiquitin-specific peptidase 12 (USP12) belongs to the ubiquitin-specific protease (USP) family of deubiquitinases that are critical for ubiquitin signaling and protein quality control. USPs function as cysteine proteases to trim or remove ubiquitin conjugates from selected substrates. Deubiquitination is important for ubiquitin signaling-mediated processes, such as regulation of protein levels or activity. Depletion of USP12 suppressed cell growth and clonogenicity and inhibited autophagy(PMID: 30266909 34997217 35898171).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP12 antibody 12608-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.
| ||
