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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP14 Recombinant antibody (82128-1-RR)

    WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP14 Recombinant antibody (82128-1-RR)

    WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using USP14 Recombinant antibody (82128-1-RR)

    IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82128-1-PBS

    Affinity and Kinetic Characterization of 82128-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

USP14 Recombinant antibody, PBS Only

USP14 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 82128-1-PBS
Clone No.3G12

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82128-1-PBS targets USP14 in WB, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP14 fusion protein Ag5979 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight60 kDa
GenBank accession numberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.