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USP15 Polyclonal antibody

Name: USP15 Polyclonal antibody
Brand: Proteintech
SKU: 14354-1-AP
Rating: 5.00 (1 reviews)

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14354-1-AP

Synonyms

Ubiquitin thiolesterase 15, Ubiquitin thioesterase 15, Ubiquitin carboxyl-terminal hydrolase 15, KIAA0529, EC:3.4.19.12

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse brain using 14354-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 14354-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 14354-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14354-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14354-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14354-1-AP
IP result of anti-USP15 (IP:14354-1-AP, 4ug; Detection:14354-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human lung using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 14354-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human placenta tissue, human breast cancer tissue, human lung tissue, human ovary tumor tissue, mouse kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 18 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

14354-1-AP targets USP15 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP15 fusion protein Ag5703 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 15
Calculated molecular weight	112 kDa
Observed molecular weight	112 kDa
GenBank accession number	BC020688
Gene symbol	USP15
Gene ID (NCBI)	9958
RRID	AB_2257148
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP15 antibody 14354-1-AP	Download protocol
IHC protocol for USP15 antibody 14354-1-AP	Download protocol
IP protocol for USP15 antibody 14354-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Immunol USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses. Authors - Qiang Zou KO Validated View Article
human,mouse	WB	Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy Authors - Xiangling Xiao KD Validated View Article
mouse	WB	Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages Authors - Teodorus Theo Susanto View Article
human	WB	Autophagy USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. Authors - Kaifeng Niu KD Validated View Article
mouse	WB	Autophagy LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a. Authors - Haihai Liang View Article
human	WB	Leukemia The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia Authors - Madeline Niederkorn View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Amy (Verified Customer) (11-08-2023)

Clean band for WB, used at a dilution lower than in the data sheet for U2OS cell lysates (1:500).

Applications: Western Blot
Primary Antibody Dilution: 1:500
Cell Tissue Type: U2OS

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP15 Polyclonal antibody

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of mouse brain using 14354-1-AP

WB analysis using 14354-1-AP

WB analysis using 14354-1-AP

WB analysis of HEK-293 using 14354-1-AP

WB analysis of HeLa using 14354-1-AP

IP experiment of mouse brain using 14354-1-AP

IHC staining of human breast cancer using 14354-1-AP

IHC staining of human breast cancer using 14354-1-AP

IHC staining of human lung using 14354-1-AP

IHC staining of mouse kidney using 14354-1-AP

IHC staining of rat small intestine using 14354-1-AP

IHC staining of human placenta using 14354-1-AP

IHC staining of human ovary tumor using 14354-1-AP

IHC staining of human ovary tumor using 14354-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.

The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia

Reviews