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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis of 4T1 using 67557-1-Ig (same clone as 67557-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IF Staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

USP15 Monoclonal antibody, PBS Only

USP15 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67557-1-PBS
Clone No.1C9G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67557-1-PBS targets USP15 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP15 fusion protein Ag5712 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 15
Calculated molecular weight 112 kDa
Observed molecular weight 112 kDa
GenBank accession numberBC020688
Gene Symbol USP15
Gene ID (NCBI) 9958
RRIDAB_2882771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4E8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.