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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP2 Polyclonal antibody

USP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15404-1-AP

Synonyms

Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using USP2 Polyclonal antibody (15404-1-AP)

    WB analysis of mouse testis using 15404-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using USP2 Polyclonal antibody (15404-1-AP)

    WB analysis of MCF-7 using 15404-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using USP2 Polyclonal antibody (15404-1-AP)

    WB analysis of mouse brain using 15404-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using USP2 Polyclonal antibody (15404-1-AP)

    IHC staining of human liver using 15404-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using USP2 Polyclonal antibody (15404-1-AP)

    IHC staining of human liver using 15404-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP2 Polyclonal antibody (15404-1-AP)

    IF Staining of HepG2 using 15404-1-AP

    Immunofluorescent analysis of HepG2 cells, using USP2 antibody 15404-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse testis tissue, MCF-7 cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15404-1-AP targets USP2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP2 fusion protein Ag7658 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 2
Calculated molecular weight 68 kDa
Observed molecular weight 41-50 kDa, 68 kDa
GenBank accession numberBC002854
Gene symbol USP2
Gene ID (NCBI) 9099
RRIDAB_10596634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing with the MW of 68, 41, 40 and 45 kDa. USP2 can exsit as a homooligomer.

Protocols

Product Specific Protocols
WB protocol for USP2 antibody 15404-1-APDownload protocol
IHC protocol for USP2 antibody 15404-1-APDownload protocol
IF protocol for USP2 antibody 15404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Transl Oncol

The role of deubiquitinase USP2 in driving bladder cancer progression by stabilizing EZH2 to epigenetically silence SOX1 expression

Authors - Fanghua Xu
  • KD Validated
View Article