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- KD/KO Validated
USP21 Polyclonal antibody
USP21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 17856-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells, recombinant ptotein, HepG2 cells, HuH-7 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
17856-1-AP targets USP21 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP21 fusion protein Ag12316 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 21 |
| Calculated molecular weight | 565 aa, 63 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC090946 |
| Gene symbol | USP21 |
| Gene ID (NCBI) | 27005 |
| RRID | AB_2878453 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP21 antibody 17856-1-AP | Download protocol |
| IHC protocol for USP21 antibody 17856-1-AP | Download protocol |
| IF protocol for USP21 antibody 17856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer | ||
Int J Mol Sci PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study. | ||
Cell Death Dis The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.
| ||
iScience TET2 stabilized by deubiquitinase USP21 ameliorates cigarette smoke-induced apoptosis in airway epithelial cells | ||
J Virol Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.
|
