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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP25 Polyclonal antibody (12199-1-AP)

    WB analysis using 12199-1-AP (same clone as 12199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using USP25 Polyclonal antibody (12199-1-AP)

    WB analysis of mouse cerebellum using 12199-1-AP (same clone as 12199-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of RAW 264.7 cells using USP25 Polyclonal antibody (12199-1-AP)

    IP experiment of RAW 264.7 using 12199-1-AP (same clone as 12199-1-PBS)

    IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using USP25 Polyclonal antibody (12199-1-AP)

    IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using USP25 Polyclonal antibody (12199-1-AP)

    IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

USP25 Polyclonal antibody, PBS Only

USP25 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12199-1-PBS targets USP25 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP25 fusion protein Ag2837 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 25
Calculated molecular weight 125 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC015930
Gene Symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2212771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHP3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.