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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP25 Polyclonal antibody

USP25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28817-1-AP

Synonyms

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using USP25 Polyclonal antibody (28817-1-AP)

    WB analysis of HEK-293T using 28817-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using USP25 Polyclonal antibody (28817-1-AP)

    WB analysis of PC-3 using 28817-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using USP25 Polyclonal antibody (28817-1-AP)

    WB analysis of RAW 264.7 using 28817-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using USP25 Polyclonal antibody (28817-1-AP)

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using USP25 Polyclonal antibody (28817-1-AP)

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using USP25 Polyclonal antibody (28817-1-AP)

    IHC staining of human lung cancer using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, PC-3 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28817-1-AP targets USP25 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP25 fusion protein Ag30463 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 25
Calculated molecular weight 125 kDa
Observed molecular weight122-138 kDa
GenBank accession numberBC015930
Gene symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2918207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as a deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

Protocols

Product Specific Protocols
WB protocol for USP25 antibody 28817-1-APDownload protocol
IHC protocol for USP25 antibody 28817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols