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  • KD/KO Validated

USP3 Polyclonal antibody

USP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12490-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

UBP, Ubiquitin-specific-processing protease 3, Ubiquitin thioesterase 3, Ubiquitin carboxyl-terminal hydrolase 3, SIH003

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, K-562 cells, RAW264.7, HepG2 cells, Jurkat cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12490-1-AP targets USP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP3 fusion protein Ag3085 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 3
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight 53-59 kDa
GenBank accession numberBC018113
Gene Symbol USP3
Gene ID (NCBI) 9960
RRIDAB_10639042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6I4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP3 antibody 12490-1-APDownload protocol
IHC protocol for USP3 antibody 12490-1-APDownload protocol
IF protocol for USP3 antibody 12490-1-APDownload protocol
IP protocol for USP3 antibody 12490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Death Differ

N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors

Authors - Zhishuang Gao
humanWB

EMBO J

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.

Authors - Doowon Huh
humanWB

BMC Med

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Authors - Zhijian Zheng
humanWB,IHC,IF

Cell Death Dis

USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer

Authors - Sheng Li
  • KD Validated
ratWB

Cell Rep

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

Authors - Kellen D Winden
humanWB

BMC Cancer

Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma.

Authors - Ke Shi
  • KD Validated
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