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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP30 Polyclonal antibody

USP30 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15402-1-AP

Synonyms

Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP30 Polyclonal antibody (15402-1-AP)

    WB analysis using 15402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15402-1-AP (USP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP30 Polyclonal antibody (15402-1-AP)

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP30 Polyclonal antibody (15402-1-AP)

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP30 Polyclonal antibody (15402-1-AP)

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP30 Polyclonal antibody (15402-1-AP)

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using USP30 Polyclonal antibody (15402-1-AP)

    IF Staining of A549 using 15402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 15402-1-AP (USP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inDaudi cells, HepG2 cells, hTERT-RPE1 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15402-1-AP targets USP30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP30 fusion protein Ag7620 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 30
Calculated molecular weight58.5 kDa
Observed molecular weight 59-67 kDa
GenBank accession numberBC004868
Gene symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

Protocols

Product Specific Protocols
WB protocol for USP30 antibody 15402-1-APDownload protocol
IHC protocol for USP30 antibody 15402-1-APDownload protocol
IF protocol for USP30 antibody 15402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Biochem Biophys Res Commun

USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury

Authors - Yang Wu
View Article