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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP33 Polyclonal antibody

USP33 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12060-2-AP

Synonyms

Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using USP33 Polyclonal antibody (12060-2-AP)

    WB analysis of HEK-293 using 12060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12060-2-AP (USP33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using USP33 Polyclonal antibody (12060-2-AP)

    IHC staining of human medulloblastoma using 12060-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12060-2-AP (USP33 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using USP33 Polyclonal antibody (12060-2-AP)

    IHC staining of human prostate cancer using 12060-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12060-2-AP (USP33 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman medulloblastoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12060-2-AP targets USP33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP33 fusion protein Ag2695 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 33
Calculated molecular weight 942 aa, 107 kDa
Observed molecular weight 107 kDa/ 103 kDa/ 93 kDa
GenBank accession numberBC016663
Gene symbol USP33
Gene ID (NCBI) 23032
RRIDAB_2213235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP33 antibody 12060-2-APDownload protocol
IHC protocol for USP33 antibody 12060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols