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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01703-3

    Sandwich ELISA standard curve of MP01703-3

    Sandwich ELISA standard curve of MP01703-3, Human USP33 Recombinant Matched Antibody Pair - PBS only. 84958-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14268. 84958-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01703-3

    Sample test of MP01703-3

    The mean USP33 concentration was determined to be 8.27 ng/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load, 4.70 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 3.49 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP33 Recombinant antibody (84958-1-RR)

    IF Staining of HeLa using 84958-1-RR (same clone as 84958-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP33 antibody (84958-1-RR, Clone: 242535A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84958-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01703-2

    Cytometric bead array standard curve of MP01703-2

    Cytometric bead array standard curve of MP01703-2, USP33 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84958-2-PBS. Detection antibody: 84958-1-PBS. Standard: Ag14268. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01703-1

    Cytometric bead array standard curve of MP01703-1

    Cytometric bead array standard curve of MP01703-1, USP33 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84958-1-PBS. Detection antibody: 84958-3-PBS. Standard: Ag14268. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84958-1-RR

    Affinity and Kinetic Characterization of 84958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84958-1-RR against Human USP33 were performed. The affinity constant is below 1 pM.

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USP33 Recombinant antibody, PBS Only (Capture/Detector)

USP33 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84958-1-PBS
Clone No.242535A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1, EC:3.4.19.12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84958-1-PBS targets USP33 as part of a matched antibody pair:

MP01703-1: 84958-1-PBS capture and 84958-3-PBS detection (validated in Cytometric bead array)

MP01703-2: 84958-2-PBS capture and 84958-1-PBS detection (validated in Cytometric bead array)

MP01703-3: 84958-1-PBS capture and 84958-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP33 fusion protein Ag14268 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 33
Calculated molecular weight 942 aa, 107 kDa
GenBank accession numberBC016663
Gene Symbol USP33
Gene ID (NCBI) 23032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TEY7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.