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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis using 68165-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis using 68165-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis of HeLa using 68165-1-Ig

    WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of Jurkat cells using USP36 Monoclonal antibody (68165-1-Ig)

    IP experiment of Jurkat using 68165-1-Ig

    IP result of anti-USP36 (IP:68165-1-Ig, 4ug; Detection:68165-1-Ig 1:3000) with Jurkat cells lysate 2030 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP36 Monoclonal antibody (68165-1-Ig)

    IF Staining of HeLa using 68165-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP36 antibody (68165-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • Flow cytometry (FC) experiment of U2OS cells using USP36 Monoclonal antibody (68165-1-Ig)

    FC experiment of U2OS using 68165-1-Ig

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug USP36 Monoclonal antibody (68165-1-Ig, Clone:1A1B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • KD/KO Validated

USP36 Monoclonal antibody

USP36 Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 68165-1-Ig
Clone No.1A1B3
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, K-562 cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68165-1-Ig targets USP36 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP36 fusion protein Ag6426 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 36
Calculated molecular weight 1123 aa, 123 kDa
Observed molecular weight150 kDa
GenBank accession numberBC038983
Gene Symbol USP36
Gene ID (NCBI) 57602
RRIDAB_2923684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)

Protocols

Product Specific Protocols
WB protocol for USP36 antibody 68165-1-IgDownload protocol
IF protocol for USP36 antibody 68165-1-IgDownload protocol
IP protocol for USP36 antibody 68165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3

Authors - Gengchen Xie
View Article