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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP4 Polyclonal antibody

USP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24976-1-AP

Synonyms

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using USP4 Polyclonal antibody (24976-1-AP)

    WB analysis of THP-1 using 24976-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 24976-1-AP (USP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using USP4 Polyclonal antibody (24976-1-AP)

    IHC staining of human ovary cancer using 24976-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24976-1-AP (USP4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24976-1-AP targets USP4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP4 fusion protein Ag21684 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Calculated molecular weight 963 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC125131
Gene symbol USP4
Gene ID (NCBI) 7375
RRIDAB_3085764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

Protocols

Product Specific Protocols
WB protocol for USP4 antibody 24976-1-APDownload protocol
IHC protocol for USP4 antibody 24976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols