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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP4 Polyclonal antibody

USP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24976-1-AP

Synonyms

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using USP4 Polyclonal antibody (24976-1-AP)

    WB analysis of THP-1 using 24976-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 24976-1-AP (USP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using USP4 Polyclonal antibody (24976-1-AP)

    IHC staining of human ovary cancer using 24976-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24976-1-AP (USP4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24976-1-AP targets USP4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP4 fusion protein Ag21684 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Calculated molecular weight 963 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC125131
Gene symbol USP4
Gene ID (NCBI) 7375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

Protocols

Product Specific Protocols
WB protocol for USP4 antibody 24976-1-APDownload protocol
IHC protocol for USP4 antibody 24976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols