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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP4 Recombinant antibody

USP4 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240797B11

Cat no : 83734-2-RR

Synonyms

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP4 Recombinant antibody (83734-2-RR)

    WB analysis using 83734-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83734-2-RR (USP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using USP4 Recombinant antibody (83734-2-RR)

    IHC staining of human ovary cancer using 83734-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83734-2-RR (USP4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of U-251 cells using USP4 Recombinant antibody (83734-2-RR)

    FC experiment of U-251 using 83734-2-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug USP4 Recombinant antibody (83734-2-RR, Clone:240797B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using USP4 Recombinant antibody (83734-2-RR)

    FC experiment of HeLa using 83734-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP4 Recombinant antibody (83734-2-RR, Clone:240797B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83734-2-RR

    Affinity and Kinetic Characterization of 83734-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83734-2-RR against Human USP4 were performed. The affinity constant is 0.598 nM.

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Tested Applications

Positive WB detected inTHP-1 cells, NCI-H1299 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83734-2-RR is USP4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP4 fusion protein Ag28297 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Calculated molecular weight 963 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC125131
Gene symbol USP4
Gene ID (NCBI) 7375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

Protocols

Product Specific Protocols
WB protocol for USP4 antibody 83734-2-RRDownload protocol
IHC protocol for USP4 antibody 83734-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols