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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP40 Polyclonal antibody

USP40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27217-1-AP

Synonyms

USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP40 Polyclonal antibody (27217-1-AP)

    WB analysis using 27217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27217-1-AP (USP40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using USP40 Polyclonal antibody (27217-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using USP40 Polyclonal antibody (27217-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27217-1-AP targets USP40 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP40 fusion protein Ag25976 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 40
Observed molecular weight140 kDa
GenBank accession numberBC013305
Gene symbol USP40
Gene ID (NCBI) 55230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP40 (ubiquitin specific peptidase 40), also known as ubiquitin thioesterase 40, deubiquitinating enzyme 40 or ubiquitin carboxyl-terminal hydrolase 40, is a 1235 amino acid protein that belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. USP40 transcript is ubiquitously expressed but abundant in the kidney, heart, spleen, liver, and testis. Within the kidney, USP40 is expressed in glomerular endothelial cells during development (PMID:28148530).

Protocols

Product Specific Protocols
WB protocol for USP40 antibody 27217-1-APDownload protocol
IHC protocol for USP40 antibody 27217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols