• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP42 Polyclonal antibody

USP42 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18811-1-AP

Synonyms

USP42

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using USP42 Polyclonal antibody (18811-1-AP)

    WB analysis of mouse brain using 18811-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18811-1-AP (USP42 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using USP42 Polyclonal antibody (18811-1-AP)

    WB analysis of mouse brain using 18811-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18811-1-AP (USP42 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USP42 Polyclonal antibody (18811-1-AP)

    IHC staining of mouse brain using 18811-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18811-1-AP (USP42 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USP42 Polyclonal antibody (18811-1-AP)

    IHC staining of mouse brain using 18811-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18811-1-AP (USP42 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18811-1-AP targets USP42 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 42
Calculated molecular weight 145 kDa
Observed molecular weight 146 kDa
GenBank accession numberBC136656
Gene symbol USP42
Gene ID (NCBI) 84132
RRIDAB_10603481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP42(ubiquitin carboxyl-terminal hydrolase 42) is also named as deubiquitinating enzyme 42 and belongs to the peptidase C19 family. It consists of 1,324 amino acids with a predicted molecular weight of 146 kDa and contains the conserved Cys, Asp (I), His and Asn/Asp (II) domains defined as one of characteristics for deubiquitinating enzymes, which may play an important role in mouse embryogenesis and spermatogenesis(PMID:16904385). This protein has 2 isoforms produced by alternative splicing. This antibody is specific to USP42.

Protocols

Product Specific Protocols
WB protocol for USP42 antibody 18811-1-APDownload protocol
IHC protocol for USP42 antibody 18811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

Authors - Shuyan Liu
View Article