Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18811-1-AP targets USP42 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 42 |
| Calculated molecular weight | 145 kDa |
| Observed molecular weight | 146 kDa |
| GenBank accession number | BC136656 |
| Gene Symbol | USP42 |
| Gene ID (NCBI) | 84132 |
| RRID | AB_10603481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9J4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP42(ubiquitin carboxyl-terminal hydrolase 42) is also named as deubiquitinating enzyme 42 and belongs to the peptidase C19 family. It consists of 1,324 amino acids with a predicted molecular weight of 146 kDa and contains the conserved Cys, Asp (I), His and Asn/Asp (II) domains defined as one of characteristics for deubiquitinating enzymes, which may play an important role in mouse embryogenesis and spermatogenesis(PMID:16904385). This protein has 2 isoforms produced by alternative splicing. This antibody is specific to USP42.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP42 antibody 18811-1-AP | Download protocol |
| IHC protocol for USP42 antibody 18811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis. |