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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using USP44 Polyclonal antibody (15521-1-AP)

    WB analysis of human placenta using 15521-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15521-1-AP (USP44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using USP44 Polyclonal antibody (15521-1-AP)

    WB analysis of mouse testis using 15521-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15521-1-AP (USP44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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USP44 Polyclonal antibody

USP44 Polyclonal Antibody for WB, ELISA
Cat No. 15521-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Deubiquitinating enzyme 44, Ubiquitin thiolesterase 44, USP44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15521-1-AP targets USP44 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP44 fusion protein Ag7888 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 44
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC030704
Gene Symbol USP44
Gene ID (NCBI) 84101
RRIDAB_2213688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0E7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP44(Ubiquitin carboxyl-terminal hydrolase 44) belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. It has a ZnF-UBP (zinc-finger ubiquitin-specific protease) domain and conserved cysteine, histidine and asparagine/aspartic acid residues characteristic of deubiquitinating enzymes. And USP44 can cleave ubiquitin from ubiquitinated substrates both in vitro and in vivo. The expression of endogenous mouse or human USP44 is very low. A localization study of USP44 showed its predominant expression in the nucleus(PMID:20402667).

Protocols

Product Specific Protocols
WB protocol for USP44 antibody 15521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Clin Invest

USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis.

Authors - Zhang Ying Y
View Article
human

Nucleic Acids Res

USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.

Authors - Xia Ting
View Article