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KD/KO Validated

USP46 Polyclonal antibody

Name: USP46 Polyclonal antibody
Brand: Proteintech
SKU: 13502-1-AP

USP46 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13502-1-AP

Synonyms

Ubiquitin-specific-processing protease 46, Ubiquitin thiolesterase 46, Ubiquitin thioesterase 46, Ubiquitin carboxyl-terminal hydrolase 46, EC:3.4.19.12

Validation Data Gallery

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AllWBIHC

WB analysis of human kidney using 13502-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 13502-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human renal cell carcinoma using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human renal cell carcinoma using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human renal cell carcinoma using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human renal cell carcinoma using 13502-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 13502-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human kidney tissue, mouse pancreas tissue
Positive IHC detected in	human renal cell carcinoma tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

13502-1-AP targets USP46 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP46 fusion protein Ag4330 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 46
Calculated molecular weight	366 aa, 42 kDa
Observed molecular weight	38-42 kDa
GenBank accession number	BC037574
Gene symbol	USP46
Gene ID (NCBI)	64854
RRID	AB_10642951
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for USP46 antibody 13502-1-AP	Download protocol
IHC protocol for USP46 antibody 13502-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IP	Nat Commun The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling Authors - Victoria H Ng View Article
mouse	WB	J Exp Med USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses. Authors - Zhongxia Yu KD Validated View Article
human	WB,IP,IF,CoIP	Biochim Biophys Acta Mol Basis Dis USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis Authors - Shun Gao View Article
mouse	WB,IF	Mol Neurobiol Ceftriaxone Modulates Ubiquitination of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole Propionic Acid Receptors to Improve Long-Term Potentiation Impairment Induced by Exogenous β-Amyloid in a Glutamate Transporter-1 Dependent Manner Authors - Rui Jiang View Article
human	WB,IHC,CoIP,IF	Exp Cell Res Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma. Authors - Maoqing Tian KD Validated View Article
human	WB	FEBS Open Bio Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins Authors - Kazuma Yoshioka View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP46 Polyclonal antibody

USP46 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of human kidney using 13502-1-AP

WB analysis of mouse pancreas using 13502-1-AP

IHC staining of human pancreas using 13502-1-AP

IHC staining of human renal cell carcinoma using 13502-1-AP

IHC staining of human renal cell carcinoma using 13502-1-AP

IHC staining of human pancreas using 13502-1-AP

IHC staining of human pancreas using 13502-1-AP

IHC staining of human renal cell carcinoma using 13502-1-AP

IHC staining of human renal cell carcinoma using 13502-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling

USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.

USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis

Ceftriaxone Modulates Ubiquitination of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole Propionic Acid Receptors to Improve Long-Term Potentiation Impairment Induced by Exogenous β-Amyloid in a Glutamate Transporter-1 Dependent Manner

Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma.

Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins