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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

    WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of MDA-MB-453s using 15158-1-AP (same clone as 15158-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of rat brain using 15158-1-AP (same clone as 15158-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of rat lung using 15158-1-AP (same clone as 15158-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of human brain using 15158-1-AP (same clone as 15158-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of human brain using 15158-1-AP (same clone as 15158-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

    IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15158-1-PBS targets USP5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP5 fusion protein Ag7252 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight 96 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
RRIDAB_2213856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45974
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.