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  • Featured Product
  • KD/KO Validated

USP50 Polyclonal antibody

USP50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24817-1-AP

Synonyms

USP50

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using USP50 Polyclonal antibody (24817-1-AP)

    WB analysis of mouse testis using 24817-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24817-1-AP (USP50 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using USP50 Polyclonal antibody (24817-1-AP)

    IHC staining of human hepatocirrhosis using 24817-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USP50 Polyclonal antibody (24817-1-AP)

    IHC staining of mouse testis using 24817-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USP50 Polyclonal antibody (24817-1-AP)

    IHC staining of mouse testis using 24817-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using USP50 Polyclonal antibody (24817-1-AP)

    IHC staining of human hepatocirrhosis using 24817-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman hepatocirrhosis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24817-1-AP targets USP50 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP50 fusion protein Ag19337 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 50
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight40 kDa
GenBank accession numberBC146493
Gene symbol USP50
Gene ID (NCBI) 373509
RRIDAB_2923574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function by targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.

Protocols

Product Specific Protocols
WB protocol for USP50 antibody 24817-1-APDownload protocol
IHC protocol for USP50 antibody 24817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Front Immunol

USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis

Authors - Chenye Zhao
  • KD Validated
View Article