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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP53 Recombinant antibody, PBS Only (Capture)

USP53 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240971A6

Cat no : 83846-1-PBS

Synonyms

KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP53 Recombinant antibody (83846-1-RR)

    WB analysis using 83846-1-RR (same clone as 83846-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83846-1-RR (USP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USP53 Recombinant antibody (83846-1-RR)

    IHC staining of mouse testis using 83846-1-RR (same clone as 83846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using USP53 Recombinant antibody (83846-1-RR)

    IHC staining of rat liver using 83846-1-RR (same clone as 83846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00821-1

    Cytometric bead array standard curve of MP00821-1

    Cytometric bead array standard curve of MP00821-1, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-2-PBS. Standard: Ag28923. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00821-2

    Cytometric bead array standard curve of MP00821-2

    Cytometric bead array standard curve of MP00821-2, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-3-PBS. Standard: Ag28923. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83846-1-RR

    Affinity and Kinetic Characterization of 83846-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83846-1-RR against Human USP53 were performed. The affinity constant is 0.878 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83846-1-PBS targets USP53 as part of a matched antibody pair:

MP00821-1: 83846-1-PBS capture and 83846-2-PBS detection (validated in Cytometric bead array)

MP00821-2: 83846-1-PBS capture and 83846-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP53 fusion protein Ag28923 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 53
Calculated molecular weight 121 kDa
Observed molecular weight120-130 kDa
GenBank accession numberNM_019050
Gene symbol USP53
Gene ID (NCBI) 54532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin specific peptidase 53 (USP53) was originally discovered in cholestasis, and its tumor suppressive effects have recently been reported in various cancers. Zhao et al. showed that USP53 interacted with FKBP51 in lung adenocarcinoma cells, and induced apoptosis via the AKT pathway. Furthermore, USP53 inhibited the progression of clear cell renal cell carcinoma by suppressing the nuclear factor κB (NF‐κB) pathway. (PMID: 35654790)