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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP53 Recombinant antibody

USP53 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240971A6

Cat no : 83846-1-RR

Synonyms

KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP53 Recombinant antibody (83846-1-RR)

    WB analysis using 83846-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83846-1-RR (USP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using USP53 Recombinant antibody (83846-1-RR)

    IHC staining of mouse testis using 83846-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using USP53 Recombinant antibody (83846-1-RR)

    IHC staining of rat liver using 83846-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83846-1-RR

    Affinity and Kinetic Characterization of 83846-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83846-1-RR against Human USP53 were performed. The affinity constant is 0.878 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, A431 cells, HeLa cells, HepG2 cells
Positive IHC detected inrat liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83846-1-RR is USP53 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP53 fusion protein Ag28923 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 53
Calculated molecular weight 121 kDa
Observed molecular weight120-130 kDa
GenBank accession numberNM_019050
Gene symbol USP53
Gene ID (NCBI) 54532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin specific peptidase 53 (USP53) was originally discovered in cholestasis, and its tumor suppressive effects have recently been reported in various cancers. Zhao et al. showed that USP53 interacted with FKBP51 in lung adenocarcinoma cells, and induced apoptosis via the AKT pathway. Furthermore, USP53 inhibited the progression of clear cell renal cell carcinoma by suppressing the nuclear factor κB (NF‐κB) pathway. (PMID: 35654790)

Protocols

Product Specific Protocols
WB protocol for USP53 antibody 83846-1-RRDownload protocol
IHC protocol for USP53 antibody 83846-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols