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  • Featured Product
  • KD/KO Validated

USP7 Monoclonal antibody, PBS Only

USP7 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D7D11

Cat no : 66514-1-PBS

Synonyms

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP7 Monoclonal antibody (66514-1-Ig)

    WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP7 Monoclonal antibody (66514-1-Ig)

    WB analysis of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

    WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected HeLa cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using USP7 Monoclonal antibody (66514-1-Ig)

    WB analysis of A549 using 66514-1-Ig (same clone as 66514-1-PBS)

    WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected A549 cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP7 Monoclonal antibody (66514-1-Ig)

    WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using USP7 Monoclonal antibody (66514-1-Ig)

    IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using USP7 Monoclonal antibody (66514-1-Ig)

    IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP7 Monoclonal antibody (66514-1-Ig)

    IF Staining of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66514-1-Ig (USP7 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66514-1-PBS targets USP7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP7 fusion protein Ag25652 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 7 (herpes virus-associated)
Observed molecular weight 128 kDa
Gene symbol USP7
Gene ID (NCBI) 7874
RRIDAB_2881877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.