Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
27791-1-AP targets USP8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP8 fusion protein Ag27143 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 8 |
| Calculated molecular weight | 1118 aa, 128 kDa |
| Observed molecular weight | 128 kDa |
| GenBank accession number | BC110590 |
| Gene Symbol | USP8 |
| Gene ID (NCBI) | 9101 |
| RRID | AB_2880973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40818 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP8 antibody 27791-1-AP | Download protocol |
| IHC protocol for USP8 antibody 27791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer | ||
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. | ||
Aging Dis Exogenous H2S Promoted USP8 Sulfhydration to Regulate Mitophagy in the Hearts of db/db Mice. | ||
Arch Biochem Biophys Mitochondria-targeted Catalase induced cell malignant transformation by the downregulation of p53 protein stability via USP28/miR-200b/PP2A-Cα axis | ||
Cancer Manag Res Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.
| ||
Vet Microbiol USP8 suppresses porcine reproductive and respiratory syndrome virus replication by positively regulating MAVS mediated Ⅰ-IFN signaling |