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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP8 Recombinant antibody (83172-2-RR)

    WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1, Human USP8 Recombinant Matched Antibody Pair - PBS only. 83172-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27143. 83172-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00188-1

    Sample test of MP00188-1

    The mean USP8 concentration was determined to be 41.15 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 47.12 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of HeLa using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using USP8 Recombinant antibody (83172-2-RR)

    FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-1-PBS. Standard: Ag27143. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve of MP00188-2, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-3-PBS. Standard: Ag27143. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

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USP8 Recombinant antibody, PBS Only (Capture)

USP8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83172-2-PBS
Clone No.240016D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83172-2-PBS targets USP8 as part of a matched antibody pair:

MP00188-1: 83172-2-PBS capture and 83172-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00188-2: 83172-2-PBS capture and 83172-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP8 fusion protein Ag27143 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 8
Calculated molecular weight 1118 aa, 128 kDa
GenBank accession numberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.