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USP9X Monoclonal antibody

USP9X Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H6G11

Cat no : 68460-1-Ig

Synonyms

FAM, FAF, EC:3.4.19.12, DFFRX, Deubiquitinating enzyme FAF-X

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP9X Monoclonal antibody (68460-1-Ig)

    WB analysis using 68460-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68460-1-Ig (USP9X antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using USP9X Monoclonal antibody (68460-1-Ig)

    WB analysis of HEK-293 using 68460-1-Ig

    WB result of USP9X antibody (68460-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, Pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68460-1-Ig targets USP9X in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 9, X-linked
Calculated molecular weight 292 kDa
Observed molecular weight 260 kDa
GenBank accession numberNM_001039590
Gene symbol USP9X
Gene ID (NCBI) 8239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for USP9X antibody 68460-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols