• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

1A6/DRIM Polyclonal antibody

1A6/DRIM Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18830-1-AP

Synonyms

DRIM, NNP73, Novel nucleolar protein 73, Protein Key 1A6, UTP20

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of HeLa using 18830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of HepG2 using 18830-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of mouse ovary using 18830-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IP experiment of HeLa using 18830-1-AP

    IP result of anti-1A6/DRIM (IP:18830-1-AP, 4ug; Detection:18830-1-AP 1:800) with HeLa cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18830-1-AP (1A6/DRIM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse ovary tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18830-1-AP targets 1A6/DRIM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name UTP20, small subunit (SSU) processome component, homolog (yeast)
Calculated molecular weight 318 kDa
Observed molecular weight 310 kDa
GenBank accession numberNM_014503
Gene symbol UTP20
Gene ID (NCBI) 27340
RRIDAB_10597541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UTP20, also named as DRIM, interacts with KIAA0649.

Protocols

Product Specific Protocols
WB protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IF protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IP protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols