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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UTRN Polyclonal antibody (27948-1-AP)

    WB analysis using 27948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27948-1-AP (UTRN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using UTRN Polyclonal antibody (27948-1-AP)

    IHC staining of human colon using 27948-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27948-1-AP (UTRN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using UTRN Polyclonal antibody (27948-1-AP)

    IHC staining of human colon using 27948-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27948-1-AP (UTRN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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UTRN Polyclonal antibody

UTRN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27948-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

DMDL, DRP, DRP 1, DRP1, Dystrophin related protein 1, UTRN, utrophin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27948-1-AP targets UTRN in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UTRN fusion protein Ag27497 相同性解析による交差性が予測される生物種
Full Name utrophin
Calculated molecular weight 395 kDa
Observed molecular weight 394 kDa, 70 kDa
GenBank accession numberNM_007124
Gene Symbol UTRN
Gene ID (NCBI) 7402
RRIDAB_2881018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46939
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Utrophin is a large protein (395 kDa) which is widely expressed and most abundant in several non-skeletal muscle tissues, for example lung, intestine, embryonic neural tube, sensory ganglia, tendons and ossifying cartilages. Two alternate intronic transcripts of utrophin have been described: Up71 and Up140.

Protocols

Product Specific Protocols
WB protocol for UTRN antibody 27948-1-APDownload protocol
IHC protocol for UTRN antibody 27948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols