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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UTX Polyclonal antibody

UTX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23984-1-AP

Synonyms

Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A, UTX

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using UTX Polyclonal antibody (23984-1-AP)

    WB analysis of COLO 320 using 23984-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UTX Polyclonal antibody (23984-1-AP)

    WB analysis of HepG2 using 23984-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UTX Polyclonal antibody (23984-1-AP)

    WB analysis using 23984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23984-1-AP targets UTX in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UTX fusion protein Ag20018 相同性解析による交差性が予測される生物種
Full Name ubiquitously transcribed tetratricopeptide repeat, X chromosome
Calculated molecular weight 1401 aa, 154 kDa
Observed molecular weight154 kDa
GenBank accession numberBC093868
Gene symbol UTX
Gene ID (NCBI) 7403
RRIDAB_2935460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KDM6A, also named UTX, mediates the removal of repressive trimethylation of histone H3, it plays a central role in the regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).

Protocols

Product Specific Protocols
WB protocol for UTX antibody 23984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Med Res

Inactivation of KDM6A promotes the progression of colorectal cancer by enhancing the glycolysis

Authors - Dexiang Zhang
View Article