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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Unc119b Polyclonal antibody (26201-1-AP)

    WB analysis using 26201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDCK cells using Unc119b Polyclonal antibody (26201-1-AP)

    WB analysis of MDCK using 26201-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Unc119b Polyclonal antibody (26201-1-AP)

    WB analysis of mouse kidney using 26201-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Unc119b Polyclonal antibody (26201-1-AP)

    IF Staining of MDCK using 26201-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26201-1-AP (Unc119b antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Unc119b Polyclonal antibody

Unc119b Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26201-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine

Applications

WB, IF/ICC, ELISA

Synonyms

AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MDCK cells, mouse kidney tissue, HEK-293 cells, HeLa cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26201-1-AP targets Unc119b in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Unc119b fusion protein Ag24599 相同性解析による交差性が予測される生物種
Full Name unc-119 homolog B (C. elegans)
Calculated molecular weight 28 kDa
Observed molecular weight 28-31 kDa
GenBank accession numberBC008617
Gene Symbol Unc119b
Gene ID (NCBI) 106840
RRIDAB_2880422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8C4B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).

Protocols

Product Specific Protocols
WB protocol for Unc119b antibody 26201-1-APDownload protocol
IF protocol for Unc119b antibody 26201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols