Unc119b Polyclonal antibody
Unc119b Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Canine
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 26201-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, MDCK cells, mouse kidney tissue, HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 26201-1-AP is Unc119b Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, Canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Unc119b fusion protein Ag24599 相同性解析による交差性が予測される生物種 |
| Full Name | unc-119 homolog B (C. elegans) |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28-31 kDa |
| GenBank accession number | BC008617 |
| Gene symbol | Unc119b |
| Gene ID (NCBI) | 106840 |
| RRID | AB_2880422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Unc119b antibody 26201-1-AP | Download protocol |
| IF protocol for Unc119b antibody 26201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Francesc R (Verified Customer) (06-14-2022) | Great results by Western blot, with band of right size that disappears in CRISPR KO clones. In attached image, lanes 1-5 are HEK293T clones (three of them UNC119B-KO) and lanes 6-11 are hTERT-RPE1 clones (all containing the protein).
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