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Validation Data Gallery

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  • Western Blot (WB) analysis of human urine sample using Uromodulin Polyclonal antibody (29975-1-AP)

    WB analysis of human urine using 29975-1-AP (same clone as 29975-1-PBS)

    human urine sample were subjected to SDS PAGE followed by western blot with 29975-1-AP (Uromodulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uromodulin Polyclonal antibody (29975-1-AP)

    IHC staining of human kidney using 29975-1-AP (same clone as 29975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uromodulin Polyclonal antibody (29975-1-AP)

    IHC staining of human kidney using 29975-1-AP (same clone as 29975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Uromodulin Polyclonal antibody (29975-1-AP)

    IF Staining of mouse kidney using 29975-1-AP (same clone as 29975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Uromodulin Polyclonal antibody (29975-1-AP)

    IF Staining of mouse kidney using 29975-1-AP (same clone as 29975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29975-1-PBS in a different storage buffer formulation.

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Uromodulin Polyclonal antibody, PBS Only

Uromodulin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29975-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29975-1-PBS targets Uromodulin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uromodulin fusion protein Ag32343 相同性解析による交差性が予測される生物種
Full Name uromodulin
Calculated molecular weight 640 aa, 70 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC035975
Gene Symbol Uromodulin
Gene ID (NCBI) 7369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07911
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)