V5-tag Polyclonal antibody
V5-tag Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (1)
Applications
WB, IF, IP, CoIP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 14440-1-AP
Synonyms
"V5-tag Antibodies" Comparison
View side-by-side comparison of V5-tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 41 publications below |
| IF | See 3 publications below |
| IP | See 6 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
14440-1-AP targets V5-tag in WB, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | V5-tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2878059 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for V5-tag antibody 14440-1-AP | Download protocol |
| IP protocol for V5-tag antibody 14440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals | ||
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. | ||
Nucleic Acids Res HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Zhongwen (Verified Customer) (09-26-2023) | The V5 antibodys shows clear band in western blots. But if you use this antibody for IP, the background is so strong that it is hard for you to find your band.
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FH Nick (Verified Customer) (04-20-2022) | Product for IP was used and had to use above the recommended concentrations
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FH Ben (Verified Customer) (10-12-2021) | Worked well, we got good images from it even at a very low dilution
|
FH Daniela (Verified Customer) (01-08-2020) | In immunoblot of HeLa total cell lysate, this antibody gives several no specific bands in the negative control sample (cell lysate from no transfected cells). Whereas it doesn't recognize any specific protein in cell lysate from HeLa transfected with a plasmid for exogenous protein in frame with V5-tag. In immunofluorescence of transfected HeLa cells this antibody give a very faint signal.
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FH Erica (Verified Customer) (12-11-2019) | I used this antibody for westtern blot of V5 tagged protein and it worked very well. The signal is very strong and there isn't background. Highly recommend
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