Validation Data Gallery

Filter: AllWBIP

WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 14440-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.
IP experiment of Recombinant protein using 14440-1-AP
IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.

V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for WB, IP, ELISA

Cat No. 14440-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, IF, IP, CoIP, ChIP, RIP, ELISA

Synonyms

GKPIPNPLLGLDST, V5, V5 tag, V5-tag

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (57)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Recombinant protein
Positive IP detected in	Recombinant protein protein

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 45 publications below
IF	See 4 publications below
IP	See 7 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below
RIP	See 1 publications below

Product Information

14440-1-AP targets V5-tag in WB, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity	recombinant protein
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	V5 Tag
Calculated molecular weight	1 kDa
Gene Symbol	V5 Tag
Gene ID (NCBI)	4
RRID	AB_2878059
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Protocols

Product Specific Protocols
WB protocol for V5-tag antibody 14440-1-AP	Download protocol
IP protocol for V5-tag antibody 14440-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation Authors - Yu Sun View Article
	WB	J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. Authors - Xiaohan Gao View Article
	WB	Nat Chem Biol E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells Authors - Siqi Shen View Article
	WB	Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis Authors - Can Cheng View Article
	WB	Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals Authors - Liuke Sun View Article
	IF	Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Authors - Xianding Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

WB analysis of Recombinant protein using 14440-1-AP

IP experiment of Recombinant protein using 14440-1-AP

V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.