V5-tag Monoclonal antibody
V5-tag Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein
Applications
WB, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
CloneNo.
2B7A9
Cat no : 66007-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 18 publications below |
| IP | See 4 publications below |
| CoIP | See 3 publications below |
| RIP | See 1 publications below |
Product Information
66007-1-Ig targets V5-tag in WB, IP, CoIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | V5-tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
Publications
| Species | Application | Title |
|---|---|---|
Science Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex. | ||
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. | ||
Nat Commun K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis | ||
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Dev Cell Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tongbin (Verified Customer) (08-25-2020) | This V5 antibody detected my V5-tagged protein expressed in HEK293T cells. The band was very specific and strong.
|
FH Zehua (Verified Customer) (02-27-2019) | Works well for IB and IP.
 |
