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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VAMP2 Monoclonal antibody, PBS Only

VAMP2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7E8

Cat no : 67822-1-PBS

Synonyms

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using VAMP2 Monoclonal antibody (67822-1-Ig)

    WB analysis using 67822-1-Ig (same clone as 67822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67822-1-Ig (VAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP2 Monoclonal antibody (67822-1-Ig)

    IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP2 Monoclonal antibody (67822-1-Ig)

    IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using VAMP2 Monoclonal antibody (67822-1-Ig)

    IF Staining of U-87 MG using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using VAMP2 Monoclonal antibody (67822-1-Ig)

    IF Staining of SH-SY5Y using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67822-1-PBS targets VAMP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen VAMP2 fusion protein Ag17908 相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 2 (synaptobrevin 2)
Calculated molecular weight 13 kDa
Observed molecular weight19 kDa
GenBank accession numberBC002737
Gene symbol VAMP2
Gene ID (NCBI) 6844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).