Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse testis using 10738-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of SH-SY5Y using 10738-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10738-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10738-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IP experiment of mouse brain using 10738-1-AP
IP result of anti-VAMP4 (IP:10738-1-AP, 4ug; Detection:10738-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of mouse brain using 10738-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10738-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10738-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10738-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 10738-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using VAMP4 antibody (10738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

VAMP4 Polyclonal antibody

VAMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10738-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Vesicle-associated membrane protein 4, VAMP-4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, mouse brain tissue, SH-SY5Y cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10738-1-AP targets VAMP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VAMP4 fusion protein Ag1090 相同性解析による交差性が予測される生物種
Full Name	vesicle-associated membrane protein 4
Calculated molecular weight	16 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC007019
Gene Symbol	VAMP4
Gene ID (NCBI)	8674
RRID	AB_2212788
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75379
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Protocols

Product Specific Protocols
WB protocol for VAMP4 antibody 10738-1-AP	Download protocol
IHC protocol for VAMP4 antibody 10738-1-AP	Download protocol
IF protocol for VAMP4 antibody 10738-1-AP	Download protocol
IP protocol for VAMP4 antibody 10738-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,rat	WB,IF,IP	J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes Authors - Min Li KO Validated View Article
human	WB	J Biol Chem Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin. Authors - Wulin Aerbajinai View Article
human	WB	Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Authors - Nazma Malik View Article
rat	WB	Biophys Rep PMID: 30596141 Authors - Min Li View Article
mouse	IF	J Biol Chem Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth. Authors - Tao Tao View Article
human	WB	J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome Authors - Hana Nůsková View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 10738-1-AP

WB analysis of SH-SY5Y using 10738-1-AP

WB analysis of mouse brain using 10738-1-AP

WB analysis of mouse brain using 10738-1-AP

IP experiment of mouse brain using 10738-1-AP

IHC staining of mouse brain using 10738-1-AP

IHC staining of mouse brain using 10738-1-AP

IHC staining of rat brain using 10738-1-AP

IHC staining of rat brain using 10738-1-AP

IF Staining of SH-SY5Y using 10738-1-AP

VAMP4 Polyclonal antibody

VAMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

PMID: 30596141

Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome