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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis of mouse skeletal muscle using 15275-1-AP (same clone as 15275-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis of human kidney using 15275-1-AP (same clone as 15275-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis of human brain using 15275-1-AP (same clone as 15275-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VAPA Polyclonal antibody (15275-1-AP)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using VAPA Polyclonal antibody (15275-1-AP)

    IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of human liver cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of human liver cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of mouse heart using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using VAPA Polyclonal antibody (15275-1-AP)

    IHC staining of mouse heart using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VAPA Polyclonal antibody (15275-1-AP)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VAPA Polyclonal antibody (15275-1-AP)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VAPA Polyclonal antibody (15275-1-AP)

    IF Staining of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 15275-1-AP (VAPA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

VAPA Polyclonal antibody, PBS Only

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15275-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15275-1-PBS targets VAPA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPA fusion protein Ag7393 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight 28 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2256991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0L0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.