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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VAPA Monoclonal antibody, PBS Only

VAPA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F10F9

Cat no : 67845-1-PBS

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAPA Monoclonal antibody (67845-1-Ig)

    WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67845-1-PBS targets VAPA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPA fusion protein Ag7819 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002992
Gene symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2918607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.