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VARS2 Polyclonal antibody

Name: VARS2 Polyclonal antibody
Brand: Proteintech
SKU: 15776-1-AP

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15776-1-AP

Synonyms

KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15776-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15776-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15776-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15776-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15776-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 15776-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15776-1-AP
IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug.
IHC staining of human liver cancer using 15776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15776-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15776-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15776-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15776-1-AP (VARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15776-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human liver tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VARS2 fusion protein Ag8460 相同性解析による交差性が予測される生物種
Full Name	valyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight	119 kDa, 57 kDa
Observed molecular weight	118 kDa
GenBank accession number	BC008844
Gene symbol	VARS2
Gene ID (NCBI)	57176
RRID	AB_2272699
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.

Protocols

Product Specific Protocols
WB protocol for VARS2 antibody 15776-1-AP	Download protocol
IHC protocol for VARS2 antibody 15776-1-AP	Download protocol
IF protocol for VARS2 antibody 15776-1-AP	Download protocol
IP protocol for VARS2 antibody 15776-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	EMBO Mol Med The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. Authors - Elena Perli View Article
human	IHC	Int J Mol Sci VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation. Authors - Elham Kayvanpour KD Validated View Article
human	WB	Front Pediatr Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy. Authors - Katarína Kušíková View Article
human	WB	Metab Brain Dis Neonatal encephalocardiomyopathy caused by mutations in VARS2. Authors - Fabian Baertling View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VARS2 Polyclonal antibody

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15776-1-AP

WB analysis of human brain using 15776-1-AP

WB analysis of HeLa using 15776-1-AP

WB analysis of human liver using 15776-1-AP

WB analysis of Jurkat using 15776-1-AP

WB analysis using 15776-1-AP

IP experiment of HeLa using 15776-1-AP

IHC staining of human liver cancer using 15776-1-AP

IHC staining of human liver cancer using 15776-1-AP

IHC staining of human ovary tumor using 15776-1-AP

IHC staining of human ovary tumor using 15776-1-AP

IF Staining of HeLa using 15776-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.

VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.

Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy.

Neonatal encephalocardiomyopathy caused by mutations in VARS2.