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- KD/KO Validated
VASH1 Polyclonal antibody
VASH1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 12730-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
Product Information
12730-1-AP targets VASH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VASH1 fusion protein Ag3435 相同性解析による交差性が予測される生物種 |
| Full Name | vasohibin 1 |
| Calculated molecular weight | 365 aa, 41 kDa |
| Observed molecular weight | 36 and 41 kDa |
| GenBank accession number | BC009031 |
| Gene symbol | VASH1 |
| Gene ID (NCBI) | 22846 |
| RRID | AB_2288318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VASH1, also named as vasohibin 1, is a angiogenesis inhibitor induced by the stimulation with VEGF. High expression of VASH1 has been researched in some tumors as a result of VEGF induction. It has been suggested that VASH-1 is clinically significant in bladder cancer, colorectal cancer and NSCLC. VASH-1 may be a potential marker of gastric cancer and a target for vascular targeting therapy. 12730-1-AP detects 41 kDa form secreted protein and 36 kDa form protein accumulated within the cells or pericellular milieu in SDS-PAGE.(PMID:19057892, 29042936, 29552154, 26666821)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VASH1 antibody 12730-1-AP | Download protocol |
| IF protocol for VASH1 antibody 12730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Development Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.
| ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
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