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KD/KO Validated

VASP Polyclonal antibody

Name: VASP Polyclonal antibody
Brand: Proteintech
SKU: 13472-1-AP

VASP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13472-1-AP

Synonyms

Vasodilator-stimulated phosphoprotein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13472-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 13472-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13472-1-AP
IP result of anti-VASP (IP:13472-1-AP, 4ug; Detection:13472-1-AP 1:10000) with HEK-293 cells lysate 920 ug.
IHC staining of human colon using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13472-1-AP (VASP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13472-1-AP (VASP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin cancer using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin cancer using 13472-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 13472-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VASP antibody (13472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 13472-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using VASP antibody (13472-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
FC experiment of HepG2 using 13472-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug VASP Polyclonal antibody (13472-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 13472-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, mouse spleen tissue, HEK-293 cells, Hela cells, C6 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human normal colon, human lung tissue, human skin cancer tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A549 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 6 publications below

Product Information

13472-1-AP targets VASP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VASP fusion protein Ag4266 相同性解析による交差性が予測される生物種
Full Name	vasodilator-stimulated phosphoprotein
Calculated molecular weight	380 aa, 40 kDa
Observed molecular weight	46 kDa, 50 kDa
GenBank accession number	BC038224
Gene symbol	VASP
Gene ID (NCBI)	7408
RRID	AB_2213418
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

Protocols

Product Specific Protocols
WB protocol for VASP antibody 13472-1-AP	Download protocol
IHC protocol for VASP antibody 13472-1-AP	Download protocol
IF protocol for VASP antibody 13472-1-AP	Download protocol
IP protocol for VASP antibody 13472-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Commun Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase. Authors - Thibault Courtheoux View Article
human	WB	PLoS Biol Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. Authors - Yajing Lv View Article
human	WB, IF, IHC	Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer. Authors - Kai Li KD Validated View Article
human	WB	Front Oncol High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells. Authors - Jun Wang View Article
human	WB	Oncol Rep TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway. Authors - Wen Liu KD Validated View Article
human	WB,IHC	Acta Histochem Exploring the oncogenic role of RGS19 in bladder cancer progression and prognosis Authors - Lei Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VASP Polyclonal antibody

VASP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 13472-1-AP

WB analysis of mouse spleen using 13472-1-AP

IP experiment of HEK-293 using 13472-1-AP

IHC staining of human colon using 13472-1-AP

IHC staining of human colon using 13472-1-AP

IHC staining of human lung using 13472-1-AP

IHC staining of human spleen using 13472-1-AP

IHC staining of human spleen using 13472-1-AP

IHC staining of human lung using 13472-1-AP

IHC staining of human skin cancer using 13472-1-AP

IHC staining of human skin cancer using 13472-1-AP

IF Staining of HepG2 using 13472-1-AP

IF Staining of A549 using 13472-1-AP

FC experiment of HepG2 using 13472-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.

Exploring the oncogenic role of RGS19 in bladder cancer progression and prognosis