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- KD/KO Validated
VASP Polyclonal antibody
VASP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 13472-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse spleen tissue, HEK-293 cells, Hela cells, C6 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human normal colon, human lung tissue, human skin cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
Product Information
13472-1-AP targets VASP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VASP fusion protein Ag4266 相同性解析による交差性が予測される生物種 |
| Full Name | vasodilator-stimulated phosphoprotein |
| Calculated molecular weight | 380 aa, 40 kDa |
| Observed molecular weight | 46 kDa, 50 kDa |
| GenBank accession number | BC038224 |
| Gene symbol | VASP |
| Gene ID (NCBI) | 7408 |
| RRID | AB_2213418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VASP antibody 13472-1-AP | Download protocol |
| IHC protocol for VASP antibody 13472-1-AP | Download protocol |
| IF protocol for VASP antibody 13472-1-AP | Download protocol |
| IP protocol for VASP antibody 13472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase. | ||
PLoS Biol Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. | ||
Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.
| ||
Front Oncol High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells. | ||
Oncol Rep TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.
| ||
Pathol Res Pract Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis. |
