VASP Monoclonal antibody
VASP Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B9A11
Cat no : 68431-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U2OS cells, A549 cells, A431 cells, MCF-7 cells, HeLa cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68431-1-Ig targets VASP in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | VASP fusion protein Ag17679 相同性解析による交差性が予測される生物種 |
Full Name | vasodilator-stimulated phosphoprotein |
Calculated molecular weight | 380 aa, 40 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC038224 |
Gene symbol | VASP |
Gene ID (NCBI) | 7408 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.
Protocols
Product Specific Protocols | |
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WB protocol for VASP antibody 68431-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |