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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VAT1 Monoclonal antibody (67474-1-Ig)

    WB analysis of HepG2 using 67474-1-Ig

    WB result of VAT1 antibody (67474-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAT1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using VAT1 Monoclonal antibody (67474-1-Ig)

    WB analysis using 67474-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67474-1-Ig (VAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using VAT1 Monoclonal antibody (67474-1-Ig)

    IHC staining of mouse pancreas using 67474-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67474-1-Ig (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

VAT1 Monoclonal antibody

VAT1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67474-1-Ig
Clone No.2C8A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67474-1-Ig targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAT1 fusion protein Ag29812 相同性解析による交差性が予測される生物種
Full Name vesicle amine transport protein 1 homolog (T. californica)
Calculated molecular weight 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC015041
Gene Symbol VAT1
Gene ID (NCBI) 10493
RRIDAB_2882701
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99536
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VAT1 antibody 67474-1-IgDownload protocol
IHC protocol for VAT1 antibody 67474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols