Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human ovary tumor tissue, rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
15773-1-AP targets VAX2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VAX2 fusion protein Ag8439 相同性解析による交差性が予測される生物種 |
| Full Name | ventral anterior homeobox 2 |
| Calculated molecular weight | 290 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC006336 |
| Gene Symbol | VAX2 |
| Gene ID (NCBI) | 25806 |
| RRID | AB_2878183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIW0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ventral anterior homeobox 2 (VAX2) has been reported to be specifically expressed in the ventral region of the prospective neural retina in vertebrates and plays crucial roles in regulating the proper development of the ventral region of eye (PMID: 21148184). VAX2 inactivatiin mouse leads to alterations in the establishment of a proper dorsoventral eye axis during embryonic development (PMID: 33817157).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VAX2 antibody 15773-1-AP | Download protocol |
| IHC protocol for VAX2 antibody 15773-1-AP | Download protocol |
| IP protocol for VAX2 antibody 15773-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Open Life Sci Depletion of VAX2 Restrains the Malignant Progression of Papillary Thyroid Carcinoma by Modulating ERK Signaling Pathway.
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