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VCAM-1/CD106 Polyclonal antibody

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11444-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

VCAM1, VCAM-1, V CAM 1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inTNF alpha treated HUVEC cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human spleen tissue, human placenta tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11444-1-AP targets VCAM-1/CD106 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VCAM-1/CD106 fusion protein Ag1993 相同性解析による交差性が予測される生物種
Full Name vascular cell adhesion molecule 1
Calculated molecular weight 739 aa, 81 kDa
Observed molecular weight110 kDa
GenBank accession numberBC017276
Gene Symbol VCAM-1
Gene ID (NCBI) 7412
RRIDAB_2214232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19320
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
WB protocol for VCAM-1/CD106 antibody 11444-1-APDownload protocol
IHC protocol for VCAM-1/CD106 antibody 11444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cardiovasc Res

HDAC4 regulates vascular inflammation via activation of autophagy.

Authors - Di Yang
humanIF

EBioMedicine

Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.

Authors - Peng Zhou
IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li

ACS Appl Mater Interfaces

Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression

Authors - Yingke Liu
IF

Br J Pharmacol

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Authors - Hang Su
human

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
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